Effects of supplemental dietary vitamin C on quality of semen from Nile tilapia (Oreochromis niloticus) breeders

The objective of this study was to evaluate the effects of vitamin C on growth and quality of semen from Oreochromis niloticus breeders. One hundred and sixty males were fed with different levels of vitamin C (0, 261, 599 and 942 mg/kg diet). The higher weight values were recorded for 599 (166 g) and 942 (175 g) mg of vitamin C/kg diet. Sperm motility, vigour and concentration were higher with 599 and 942 mg of vitamin C/kg diet. The semen volume, gonadosomatic index and plasma protein data from the last week showed a direct relationship with increasing levels of vitamin C. No changes were observed in the hepatosomatic index and blood glucose. The haematocrit and erythrocyte showed higher values estimated by equations derived at 850 and 638 mg vitamin C/kg diet, respectively. The leucocytes were inversely proportional to the increasing levels of vitamin C. After 100 days of feeding, animals fed the diet containing 942 mg vitamin C/kg diet had higher sperm motility, linearity, curvilinear velocity, straight line velocity and average path velocity (p < .05). Higher values of beat cross‐frequency were observed in broodfish fed diets containing 942 and 599 mg vitamin C/kg. The different vitamin C levels did not cause differences in straightness, lateral head displacement and sperm morphology. For Nile tilapia males on intensive rearing and handling conditions, vitamin C levels between 599 and 942 mg/kg may be used for a better performance and quality of semen.     

Rescue of American chestnut with extraspecific genes following its destruction by a naturalized pathogen

Following the near-obliteration of American chestnut (Castanea dentata [Marsh.] Borkh.) by the chestnut blight early in the last century, interest in its restoration has been revived by efforts to develop a blight-resistant form of the species. We summarize progress and outline future steps in two approaches: (1) a system of hybridizing with a blight-resistant chestnut species and then backcrossing repeatedly to recover the American type and (2) transformation of American chestnut with a resistance-conferring transgene followed by propagation and conventional breeding. Several decades of effort have been invested in each approach. More work remains, but results indicate that success is within practical reach. The restoration of C. dentata to its native habitat now appears to be less a matter of time and conjecture than ever before in 90 years of work by public and private entities. The difficult and protracted task of incorporating extraspecific genes for resistance into a tree species with lethal susceptibility to a naturalized pathogen represents perhaps the most extreme of restoration challenges. Its pursuit by a small non-governmental organization supported primarily by philanthropy and volunteers may serve as a model for other species threatened by exotic pathogens or insects.     

Can a problem-solving approach strengthen landscape ecology’s contribution to sustainable landscape planning?

The need to avert unacceptable and irreversible environmental change is the most urgent challenge facing society. Landscape ecology has the capacity to help address these challenges by providing spatially-explicit solutions to landscape sustainability problems. However, despite a large body of research, the real impact of landscape ecology on sustainable landscape management and planning is still limited. In this paper, we first outline a typology of landscape sustainability problems which serves to guide landscape ecologists in the problem-solving process. We then outline a formal problem-solving approach, whereby landscape ecologists can better bring about disciplinary integration, a consideration of multiple landscape functions over long time scales, and a focus on decision making. This framework explicitly considers multiple ecological objectives and socio-economic constraints, the spatial allocation of scarce resources to address these objectives, and the timing of the implementation of management actions. It aims to make explicit the problem-solving objectives, management options and the system understanding required to make sustainable landscape planning decisions. We propose that by adopting a more problem-solving approach, landscape ecologists can make a significant contribution towards realising sustainable future landscapes.     

Chemical Activation with a Combination of Ionomycin and Dehydroleucodine for Production of Parthenogenetic,ICSI and Cloned Bovine Embryos

The aim of this study was to evaluate the potential of dehydroleucodine (DhL), a new drug isolated from a medicinal herb used in Argentina, for activation of bovine oocyte. Several DhL concentrations and exposure times after ionomycin (Io) treatment were tested. The optimal DhL treatment, found for parthenogenetic development, was employed to produce bovine embryos by intracytoplasmic sperm injection (ICSI) and somatic cell nuclear transfer (SCNT). The best parthenogenic embryo developments were observed with 5 μm Io for 4 min followed by 5 μm DhL concentration and after 3‐h exposure time (52.3% cleavage; 17.4% morulae; 7.3% blastocyst; n = 109). This treatment generated no significant differences with standard Io plus 6‐dimethylaminopurine (DMAP) treatment in preimplantation embryo development. In our conditions, the embryo development reached after ICSI and SCNT assisted by the DhL treatment did not differ in terms of cleavage and blastocyst development from activation with standard Io plus DMAP treatment (p > 0.05). In conclusion, DhL utilization to activate oocytes and induce development of parthenogenotes, ICSI‐embryos or SCNT‐embryos is reported here for first time.     

Recall bias in recreational summer flounder party boat trips and angler preferences to new approaches to bag and size limits

Three innovative approaches to bag and size limits were evaluated in the recreational summer flounder Paralichthys dentatus fishery. Each approach was designed to reduce discard mortality while increasing angler satisfaction, yet still limiting recreational take within management goals. Each was compared to the 2006 legal bag and size limits on party boat trips from New Jersey and New York. Angler-specific catch data were collected during the trips, and anglers completed a questionnaire while sailing back to port. Comparison of questionnaires to observer records revealed that anglers could not accurately recall the number of fish kept or released. Anglers overestimated both kept and discarded fish by a factor of about two. Neither fishing scenario, age, sex, nor years fished significantly influenced the accuracy of survey reports of kept fish. Anglers on three of five boats over-reported landings. Reported landings were nearly accurate on two boats. Survey accuracy for reported discards was influenced by bag-and-size-limit scenario and differed among boats, sexes, and fishing experience, but no predictable pattern was evident. In particular, bias in reporting was unrelated to angler sex, age, experience, and performance on observed trips or any other criterion measured in this study. Anglers preferred the slot limit most and the 2006 legal bag and size limit least. High grading and transfer of fish among anglers were rare occurrences. Our study demonstrated that the summer flounder fishery is a consumptive fishery.     

Hemp-fiber based nonwoven composites: Effects of alkalization on sound absorption performance

The effects of the material and treatment parameters on airflow resistivity and normal-incidence sound absorption coefficient of alkalized three layered nonwoven composites have been studied. The material parameters included fiber size and porosity. The treatment factors included the temperature, duration and concentration. The alkalized composite was a three-layered nonwoven sandwich structure consisting layers of Polypropylene/Hemp/Polypropylene. Alkalization treatment has been found to result in a loss of basis weight and a decrease in air flow resistivity. Among treatment factors, only temperature was found to be a statistically-significant factor on air flow resistivity. Higher-temperature alkalization leads to higher air flow resistivity compared to the lower-temperature treatment. Alkalization at higher temperature and higher concentrations gives better results in normalized sound absorption performance compared to lower-temperature and lower-concentration treatments, respectively.     

Effects of Loma morhua (Microsporidia) infection on the cardiorespiratory performance of Atlantic cod Gadus morhua (L).

The microsporidian Loma morhua infects Atlantic cod (Gadus morhua) in the wild and in culture and results in the formation of xenomas within the gill filaments, heart and spleen. Given the importance of the two former organs to metabolic capacity and thermal tolerance, the cardiorespiratory performance of cod with a naturally acquired infection of Loma was measured during an acute temperature increase (2 °C h^?1) from 10 °C to the fish's critical thermal maximum (CT_Max). In addition, oxygen consumption and swimming performance were measured during two successive critical swimming speed (U_crit) tests at 10 °C. While Loma infection had a negative impact on cod cardiac function at warm temperatures, and on metabolic capacity in both the CT_Max and U_crit tests (i.e. a reduction of 30–40%), it appears that the Atlantic cod can largely compensate for these Loma‐induced cardiorespiratory limitations. For example, (i) CT_Max (21.0 ± 0.3 °C) and U_crit (~1.75 BL s^?1) were very comparable to those reported in previous studies using uninfected fish from the same founder population; and (ii) our data suggest that tissue oxygen extraction, and potentially the capacity for anaerobic metabolism, is enhanced in fish infected with this microsporidian.     

Sugarcane Response to DRIS-Based Fertilizer Supplements in Florida

Soil testing is the primary basis for fertilizer recommendations in Florida sugarcane ( Saccharum spp.), but it has the limitations of generally being performed only before the plant cane crop and not providing information for nitrogen or micronutrient availability. Leaf analysis is a useful diagnostic tool that can complement soil testing and may allow more cost-effective fertilizer applications for each crop. The Diagnosis and Recommendation Integrated System (DRIS) was used to determine leaf nutrient status in a study evaluating the effectiveness of a summer fertilizer supplement. There were 19, 24 and 26 paired commercial field comparisons in 2004/05, 2005/06 and 2006/07 respectively. Each field of each test pair received normally recommended fertilizer applications based on pre-plant soil tests, with one field of each pair receiving a June/July fertilizer supplement based on DRIS indices of leaf samples collected in April/May. There was no response in tonnes of sugarcane ha⁻¹ or sugar tonne cane⁻¹ to the fertilizer supplements for organic or mineral soils or for plant or first ratoon crops. A more cost-effective use of leaf analysis appears to be with the adjustment of the next amendment or fertilizer application, generally for next year's crop or at the next sugarcane planting, rather than adding an additional fertilizer supplement to the current crop.